Do not cut and pull the tissue repeatedly, causing tissue compression. 4. Positive expression of cytoplasm and cell membrane, for example, EMA can show membranous and diffuse cytoplasmic positive reactions; CD30 antibody can show both membrane and cytoplasmic spot positive reactions. The antigens we detect are diverse. At this time, a few drops of ethanol can be appropriately added to the water. Soak the detergent powder for 30 minutes, rinse, and dry. With the extension of fixed time, the detection intensity of tissue antigen will gradually decrease. 1)
Poly-L-Lysine (poly-L-lysine) 1.0ml cryo tube, 0. Wash in PBS for 5 minutes. d The room temperature is too low: below 15 degrees, it should be placed in a 37 degree incubator for 30-60 minutes, or a 4 degree refrigerator overnight. Simple formulation method: Add 1 g NaH2PO4, 15. 1. Wash 2 times in PBS for 5 minutes each time (if necessary, apply 0. The service items include: scientific and clinical grade adenovirus, lentivirus, adeno-associated virus (AAV) packaging, plasmid vector construction, TALEN gene. Wash with PBS 2-3 times for 5 minutes each. 5.0-8. (The optimal temperature is 92-95 ° C) (3) Enzymatic digestion: the precautions for this antigen repair: 1) The tissue cannot be dried. Especially during shooting, do n’t use high magnification lens for a while, and low magnification lens for a while, switch the objective lens back and forth.
Wash 3 times in PBS for 2 minutes each. The pH of the antigen retrieval solution is very important. Not suitable for long-term preservation of tissue specimens.5mol / L EDTA buffer solution (ph8. Pure acetone II, about 5 seconds. Common physical methods include simple heating, microwave processing, and high-pressure heating. For frozen sections, formaldehyde fixation is sometimes better than frozen acetone; but for different tissues and antigens, different fixative solutions can be used. Immunohistochemical staining
The S-P immunohistochemical staining kit uses a biotin-labeled secondary antibody and a streptavidin-linked peroxidase and substrate pigment mixture to determine antigens in cells and tissues. Generally at room temperature 20-28 degrees c DAB color development time is too long or the concentration is too high
https://www.mingjibio.com/product/cryo-tube/
Poly-L-Lysine (poly-L-lysine) 1.0ml cryo tube, 0. Wash in PBS for 5 minutes. d The room temperature is too low: below 15 degrees, it should be placed in a 37 degree incubator for 30-60 minutes, or a 4 degree refrigerator overnight. Simple formulation method: Add 1 g NaH2PO4, 15. 1. Wash 2 times in PBS for 5 minutes each time (if necessary, apply 0. The service items include: scientific and clinical grade adenovirus, lentivirus, adeno-associated virus (AAV) packaging, plasmid vector construction, TALEN gene. Wash with PBS 2-3 times for 5 minutes each. 5.0-8. (The optimal temperature is 92-95 ° C) (3) Enzymatic digestion: the precautions for this antigen repair: 1) The tissue cannot be dried. Especially during shooting, do n’t use high magnification lens for a while, and low magnification lens for a while, switch the objective lens back and forth.
Wash 3 times in PBS for 2 minutes each. The pH of the antigen retrieval solution is very important. Not suitable for long-term preservation of tissue specimens.5mol / L EDTA buffer solution (ph8. Pure acetone II, about 5 seconds. Common physical methods include simple heating, microwave processing, and high-pressure heating. For frozen sections, formaldehyde fixation is sometimes better than frozen acetone; but for different tissues and antigens, different fixative solutions can be used. Immunohistochemical staining
The S-P immunohistochemical staining kit uses a biotin-labeled secondary antibody and a streptavidin-linked peroxidase and substrate pigment mixture to determine antigens in cells and tissues. Generally at room temperature 20-28 degrees c DAB color development time is too long or the concentration is too high
https://www.mingjibio.com/product/cryo-tube/
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